Household hums below hearing range damage mouse kidneys in five-day experiment

Previous studies have hinted that people surrounded by more environmental noise—low, deep rumblings from air-conditioning units, elevator motors, distant traffic and ventilation ducts—tend to have worse kidney function. But these studies could not identify which part of the noise was to blame or the specific ways it might affect the kidneys.

Now, a team led by Takumi Kagawa and Masashi Kato at the Nagoya University Graduate School of Medicine has provided the first direct experimental evidence that the low-frequency component of environmental noise is responsible for adverse effects on kidney health in mice.

Their findings were published in the journal Environmental Science & Technology.

Pitch matters more than volume

Kagawa and Kato first started by recording real noise from two ordinary household machines: the outdoor unit of an air conditioner and a heat pump water heater. Using audio software, they then split each recording into two: a low-frequency version containing only sounds at or below 100 hertz and a high-frequency version containing everything above it.

Sounds were played to mice for 12 hours a day over five days, at night, when they are naturally active. The researchers then measured two standard markers of kidney health in the animals’ blood: creatinine and urea nitrogen, both of which build up when the kidneys stop filtering waste efficiently.

Playing the unedited environmental noise containing all frequencies pushed both markers up, indicating kidney damage. But when only the high-frequency noise was played, nothing happened. This meant the lower frequencies were responsible for increasing creatinine and urea nitrogen levels, as the researchers soon verified.

“The biggest surprise to me was that the low-frequency component caused kidney dysfunction even though it was below the hearing range of mice,” Kagawa said. “In contrast, the higher-frequency component did not cause kidney dysfunction, even when both had the same physical sound pressure level,” he added, implying that the frequency of noise mattered more than its loudness.

Squeezed blood vessels

To find out the mechanism, the researchers looked inside the kidneys. Kidneys clean the blood using a vast network of glomeruli—tiny knots of blood vessels that act as the organ’s filters. In the exposed mice, these filters were swollen and their delicate filtering membranes had thickened.

Additionally, the damaged kidneys were producing more endothelin-1, a molecule that can bind to receptors to clamp down on blood vessels. Under normal conditions, endothelin-1 helps regulate blood flow. But researchers suspected that low-frequency noise can push it into overdrive, causing constricted renal blood vessels.

To test this idea, the researchers gave a second group of mice ambrisentan, a drug already prescribed to patients to treat hypertension, which blocks endothelin’s effects. The treated animals kept their kidney markers closer to normal and showed much less damage to their glomeruli, confirming endothelin signaling as a key pathway.

Because this study was conducted in mice, its relevance to humans remains unknown. However, “our findings highlight the importance of considering low-frequency noise, which has often been overlooked, in future human studies on environmental noise,” Kato said.

A double-edged sword

The result is also intriguing because the same researchers have found some beneficial effects of low-frequency sound, such as reducing motion sickness and blood flow in skin blood vessels.

This suggests that “the biological effects of low-frequency sound depend on its frequency, sound level and duration of exposure,” Kato said. “Moving forward, we hope to investigate both the beneficial and adverse effects of low-frequency sound and scientifically clarify which exposure conditions are harmful and which promote health.”

Who’s behind this story?

Sadie Harley

BSc Life Sciences & Ecology. Microbiology lab background with pharmaceutical news experience in oil, gas, and renewable industries.

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Robert Egan

Bachelor’s in mathematical biology, Master’s in creative writing. Well-traveled with unique perspectives on science and language.

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