Head coach Eddie Jones has named 12 uncapped players in a much-changed England training squad for this autumn’s internationals.
Players from six of the Premiership’s 12 teams were unavailable for selection because of league commitments.
The 28-man squad will meet for a three-day training camp on Tuesday.
England face the Barbarians on 25 October, before completing the 2020 Six Nations against Italy and then playing four games in the Autumn Nations Cup.
Northampton scrum-half Alex Mitchell, Harlequins number eight Alex Dombrandt and Northampton lock David Ribbans are among the 12 uncapped players named.
“Our preparation to build the team for the Italy game starts now,” Jones said.
“We’ll be slowly building up, working on the basics and working on team cohesion. We’re excited to be back together in camp representing England.”
Players from Exeter, Wasps, Bristol, Bath, Sale and Worcester are not included in Jones’ first training squad since the pandemic paused the 2020 Six Nations in March.
Sale’s game against Worcester has been moved to Wednesday after the club recorded 19 positive coronavirus cases but there are doubts it will go ahead.
If Sharks win that game, they will make it to the play-offs instead of Bath. Exeter, Wasps and Bristol have already secured their places in the semi-finals which take place on 10 and 11 October.
Another England training squad will be announced after that, with the team meeting again on 15 October.
Bristol and Exeter have European finals to play on 16 and 17 October respectively and the Premiership final takes place on 24 October.
England play Barbarians the next day and the week after will attempt to secure the Six Nations title against Italy.
England should be back to full strength when they play their first match of the Autumn Nations Cup against Georgia at Twickenham on 14 November.
They then play Ireland and Wales, the other two teams in Group A, before facing the side in the equivalent finishing position from Group B, which contains Scotland, France, Italy and Fiji, on 6 December.
Backs: Ali Crossdale, Saracens*, Elliot Daly, Saracens, Fraser Dingwall, Northampton Saints*, Nathan Earle, Harlequins*, Owen Farrell, Saracens, George Ford, Leicester Tigers, Piers Francis, Northampton Saints, George Furbank, Northampton Saints, Willi Heinz, Gloucester Rugby, Joe Marchant, Harlequins, Jonny May, Gloucester Rugby, Alex Mitchell, Northampton Saints*, Ollie Thorley, Gloucester Rugby*, Ben Youngs, Leicester Tigers
Forwards: Jack Clement, Gloucester Rugby*, Alex Dombrandt, Harlequins*, Ellis Genge, Leicester Tigers, Jamie George, Saracens, Joe Heyes, Leicester Tigers*, Maro Itoje, Saracens, Simon Kerrod, Harlequins*, Lewis Ludlow, Gloucester Rugby*, Alex Moon, Northampton Saints*, David Ribbans, Northampton Saints*, Jack Singleton, Gloucester Rugby, Billy Vunipola, Saracens, Mako Vunipola, Saracens, Mark Wilson, Newcastle Falcons