IFI, which is the body with responsibility for freshwater fish and their habitats in Ireland, said the kill covered around a one kilometre stretch of water.
A total of 263 brown trout and 26 elver have now been collected, the IFI added.
“IFI is actively probing the cause of this harmful ecological event, but it’s too early to definitively establish how it occurred,” the agency said in a statement.
“Monitoring and assessment of the impact will continue to be undertaken to identify the cause of the fish kill, and the quality of the water”.
A number of water samples have been collected, IFI said, “to gather evidence of the discharge”.
The IFI added that a separate investigation is being carried out by officers from Donegal County Council.
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